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KMID : 1189420110240010045
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2011 Volume.24 No. 1 p.45 ~ p.63
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The Effects of Ethanol Extract from Atractylodes Chinensis Rhizome on the Mast Cell-Mediated Inflammatory Responses
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Kim Sun-Min
Kim Kyung-Jun
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Abstract
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Objective :Atractyloides Chinensis Rhizome (ACR) is widely used in oriental medicine as a remedy for an inflammation and an allergic disease. However, as yet there is no clear explanation of how ACR affects the production of inflammatory cytokine. This study was to determine the effects of ACR on the mast cell-mediated inflammatory responses.
Method:The amount of inflammatory cytokine production induced by the phorbol myristate acetate (PMA) plus calcium ionophore(A23187) in the human mast cell line (HMC-1) incubated with various concentrations of ACR was measured. The TNF-α protein levels were analysised by Western blots. The TNF-α, IL-6 and IL-8 secreted protein levels were measured by the ELISA assay. The TNF-α, IL-6 and IL-8 mRNA levels were measured by the RT-PCR analysis. NF-xB, phospho-IxB and MAPKs were examined by Western blot analysis. The NF-xB promoter activity was examined by a luciferase assay.
Results: 1. The expressions of TNF-α and TNF-α mRNA were decreased dose-dependently at 0.05-0.2mg/㎖ of ACR and significantly decreased at 0.2mg/㎖. 2. The expressions of IL-6 and IL-6 mRNA were decreased dose-dependently at 0.05-0.2mg/㎖ of ACR and significantly decreased at 0.2mg/㎖. 3. The expressions of IL-8 and IL-8 mRNA were decreased dose-dependently at 0.05-0.2mg/㎖ of ACR and significantly decreased at 0.2mg/㎖ specially. 4. The expressions of Phosphorylated-JNK were decreased, not p38, ERK 5. The expressions of NF-xB were decreased dose-dependently at 0.1-0.2mg/㎖ of ACR. The expressions of Phosphorylated IxB were significantly decreased at 0.2mg/㎖. In addition, ACR suppressed PMA plus A23187-induced NF-xB promoting activity.
Conclusion: It is suggested that ACR should suppress through inhibition of NF-xB activity and cytokine production.
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KEYWORD
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Atractylodes Chinensis Rhizome(ACR), The human mast cell line (HMC-1), MAPKs, NF-xB
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